ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (11): 1974-1986.doi: 10.11843/j.issn.0366-6964.2015.11.009

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Polymorphism of Five SNPs in Exon 10 of Growth Hormone Receptor Gene in Guizhou Pony(Equus caballus

RAN Xue-qin1* ,ZHAO Xing-yan1,WANG Jia-fu2,TIAN Song-jun3,WEI Xiao-hong3   

  1. (1.Faculty of Animal Science,Guizhou University,Guiyang 550025,China;2.Institute of Agro-Bioengineering,Guizhou University,Guiyang 550025,China;3.Bureau of Animal Husbandry and Veterinary of Ziyun of Guizhou,Ziyun 550800,China)
  • Received:2014-12-19 Online:2015-11-23 Published:2015-11-23

Abstract:

To explore the reason for the slow growth of Guizhou pony,sequences of exon 10 in GHR gene were cloned from the genome of Guizhou pony by polymerase chain reaction(PCR) and gene cloning methods.Distribution of 5 single nucleotide polymorphism(SNP) sites was determined by allele-specific PCR(AS-PCR) and single-strand conformation polymorphism(SSCP) methods.Taking Ili horse as control,the relationship between the genotypes of GHR and body sizes of Guizhou pony and Ili horse was calculated based on population genetics and bioinformatics algorithms.The results showed that a total of 10 haplotypes of exon 10 of GHR gene were sequenced from Guizhou pony,in which contained 10 SNP sites.Among them,5 sites,1028,1267,1471,1697 and 1732,changed the amino acid residues of GHR.Frequencies of 3 sites,1028,1267 and 1471,were tested by AS-PCR method.Compared with Ili horse,allele A at site 1028 was dominant and related with the lower rump height and higher cannon circumference in Guizhou pony.The percentage of allele E at 1471 site in Guizhou pony was higher than that in Ili horse and related to higher chest girth ratio of pony.Heterozygous genotypes of 1697 and 1732 sites were detected from the shortest male pony with 106 cm in height,6 years old.The V343A change resulted from 1028 site located in the ubiquitin-dependent endocytosis(UbE) motif and might affect the GHR internalization and degradation.Changes at S491G,Y566C and R578G sites resulted from SNP sites of 1471,1697 and 1732,might directly or indirectly impact the phosphorylation of GHR and influence the activation of signal transduction pathways.It suggested that the polymorphisms in exon 10 of GHR gene might have an effect on the growth and development of Guizhou pony.

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